I am validating for recovery factor in swab sampling for testing active residue in cleaning validation but I have some question in need of your assistance. Please help me clarify some following problems:
Which solvent using in swab sampling? Can I use the mobile phase of HPLC chromatography in swab sampling or only use water- ethanol - isopropanol?
Assumed that the acceptance value for active residue in my product is not more that 1.12 microgram per inch and the swab area is 100 inch. Anyone help me to experiment design for evaluate the swab recovery.
In my opinion, I performed swab recovery at 50%, 100%, 150% of acceptance value. It means I spiked 56 microgram, 112 microgram and 168 micrograp take swab sampling. Is this ok?
Thank you very much for your reply!