Can RRF be calculated by comparing the area of Impurity and area of active analyte?

If yes,then how?

[quote=annapurna.sachan]Can RRF be calculated by comparing the area of Impurity and area of active analyte?

If yes,then how?[/quote]

Dear Annapurna,

If the impurity standards are available then make linearity solutions of impurity and the prepare also same concentration solution of active analyte. Then draw straight line and find out linear correlation coefficient, slope, Y-intercept and residual sum of square. Then slop of substance divided by the slope of the impurity and multiply these value by impurity %standard , you will be find RRF value. Those impurities in which no authentic reference material is available and for unknown peaks, the RRF is assumed to be 1.00.

Thanks

Shahnawaz Sajid

[quote=Shahnawaz]Dear Annapurna,

If the impurity standards are available then make linearity solutions of impurity and the prepare also same concentration solution of active analyte. Then draw straight line and find out linear correlation coefficient, slope, Y-intercept and residual sum of square. Then slop of substance divided by the slope of the impurity and multiply these value by impurity %standard , you will be find RRF value. Those impurities in which no authentic reference material is available and for unknown peaks, the RRF is assumed to be 1.00.

Thanks

Shahnawaz Sajid[/quote]

Thanx Shahnawa,

I have read the same post before posting my question.

RRF calculation by response method is fine, but I have heard some other ways too can be used to calculate RRF. Do You have some iea.