Just wondering what is the inductry standard on the time limit before Bacillus stearothermophilus BI’s must be processed and incubated foling the end of the sterilization cycle? I have heard of some companies using 4 hours from cycle end and others 4 hours from when the autoclave door is opened.
“Place the BIs in the growth media no [COLOR=“darkred”]more than 8 hours after removal from the sterilization chamber or removal from the sterilized load of devices. Incubate all of the BIs for a minimum of 7 days[/color].”
This is FDA’s Biological Indicator Premarket Notification
Document Issued on 4th October 2007
You can refridgerate up to 24hours just make sure your procedure states so.