How To Carryout Gc Validation ,fid & Tcd With Auto Sampler What Is The Method Available & Where?
similar approach as for hplc. qualify software (typically comms testing, setup and configuration testing, access and security testing, data integrity checks, process testing, etc…). Then qualify hardware components:
GC + Headspace:
Verify accuracy of heated zones across operating range (detector, injector inlets, oven, heated valves)
Verify accuracy of flow and pressure controllers (need a manometer/flowmeter). Tested areas include make-up gas flow, carrier gas flow, ignition gas flow on FID, split flows, inlet flows/pressures, etc.
Pressure integrity/valve leak testing
Verify accuracy of oven temperature programs
Verify detector sensitivity and linearity (can use a simulator or other voltage generator)
Verify correct keypad operation (sample carousel rotation, manually programmed injection, etc)
Verify accuracy of headspace heated zones temperature controllers (sample oven, valve box, transfer line, sample loop).
Verify accuracy of headspace injection cycles
Verify headspace keypad operation
Verify performance using certified test solution (BTEX or or other certified equivalents). check response/retention time repeatability. Perform repeat injections (around 5 - 6).
For linearity test use a solution containing 5 - 7 components of increasing concentration designed to give individual peak responses between 25 mV - 500 mV/alternately 10 - 1000 nA. Perform 2 injections, average response, plot against conc/component and perform regression to determine coefficient of correlation R value.
Hope this helps…
vishwesh2007, you might also consider GC v-kit (
www.v-kit.com/gc.asp
) - a kit that contains software, procedures and traceable standards required for a GC OQ.
[quote=marwaan]similar approach as for hplc. qualify software Verify accuracy of heated zones across operating range (detector, injector inlets, oven, heated valves)
Verify accuracy of flow and pressure controllers (need a manometer/flowmeter). Tested areas include make-up gas flow, carrier gas flow, ignition gas flow on FID, split flows, inlet flows/pressures, etc.
Pressure integrity/valve leak testing
Verify accuracy of oven temperature programs
Verify detector sensitivity and linearity (can use a simulator or other voltage generator)
Verify correct keypad operation (sample carousel rotation, manually programmed injection, etc)
Verify accuracy of headspace heated zones temperature controllers (sample oven, valve box, transfer line, sample loop).
Verify accuracy of headspace injection cycles
Verify headspace keypad operation
…[/quote]
I apologize for sounding like a newbie, but how does one accurately measure the temperature of an injector or detector, which I’m assuming would be on the inside? Also, what sort of extra equipment is needed for some or all of these measurements listed above?
My company is currently revising their calibration/validation policies on all of the instrumentation, so this is a very timely question for me. So thank you in advance
Dear Charles,
Temperature verification of the injector can be done using a calibrated probe.
For detector you need to remove collector head.
For ramping verification keep RRT parameter of components in calibration standard.