Method validation

Dear all

In a validated method can i change the diluent ratio.

My procedure is as follows in sample add 25 ml diluent with shanking & than make the volume with water. i have done the validation with this method.

Now during analysis my peak is splitted & the reason for that is diluent.
I want to use 2.5 ml diluent instead of 25 ml.

Can i change this.

Anup Sharma

splitted peak is not due to diluent effect. you have got problem with your column.
if you have more question about this, please refer to chromatogprahy forum.