Leak Test for Capped Bottles/Vials

Dear all
i got some information about microbiology challenge for vial.
used Brevundimonas diminuta ATCC 19146 (or Serratia marcescens (ATCC 13880))as a
test organism with a concentration of not less than 10^5 cfu/ml for
challenge test and CCI test.

Sincerely

[quote=cdpro223]Dear all
i wanna to do a test about microbiology challenge for vial.
but i don’t know the method.choose which one Microorganism to soak.

thank you everyone.[/quote]

How do I determine the sample size for the microbial immersion testing?
Our batch size is 16000 vials.