We are manufacturing the X product. The intermediate of X product is also being manufactured by us. The cleaning validation has to de done for intermediate. The testing for detection of intermediate was performed by UV, HPLC and TLC .There is no response (Detection) in UV, HPLC and TLC .What we should do. How we ensure that the manufactured intermediate has been removed completely. The intermediate product is insoluble in water; it’s soluble in solvents only.
Please share your ideas and views.
R C Pantola