Blank is required between standard 5 injections & sample solution?

Hi Good morning to all

In assay by HPLC analytical method validation, after 5 stadard injections wether we need to do a blank or we can directly run sample preparation?

After standard no need of inject balnk unless specify in test method.However we do bracketing for sample for SST.

No need to do blank

Blank after 5 standard injections is necessary as a bracketing injection to confirm that system suitability



It not necessary to inject blank how ever ,up to some extent “yes” but it depends on the compound and the chromatographic processes.
And injecting blank is to avoid the carry over from the last injection in which the SST can be achieved