Bioanalytical Method Validation

Hi… I am involved in bio-analysis. I want to know if I use HPLC then do I need to perform “Haemolysis Effect Experiment” as a part of Method Validation? In case of LC-MS/MS we have to perform the “Haemolysis Effect”, but I am not sure for HPLC. If not the what is the reason for not performing the experiment incase of an HPLC method?:rolleyes: